检测信息
叶绿素A测定是一种用于定量分析水体、藻类及沉积物中叶绿素A含量的检测手段,广泛应用于环境监测、海洋生态评估及水质富营养化评价领域。
检测目的在于评估初级生产力及水体富营养化程度,主要涉及分光光度法、荧光法及高效液相色谱法等技术手段,以准确获取色素浓度数据。
检测流程包含样品采集、滤膜富集、冷冻保存、溶剂萃取及上机测试,核心参数涵盖吸光度值、保留时间及浓度定量结果。
检测方法
- JJF (沪苏浙皖) 4009-2023 荧光法叶绿素a测定仪校准规范
- HJ 897-2017 水质 叶绿素a的测定 分光光度法
- ISO 29841:2009 Vegetable fats and oils — Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins)
- BS EN ISO 29841:2014+A1:2016 Vegetable fats and oils. Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins)
- DIN EN 18034:2025-07 Algae and algae products - Methods of sampling and analysis - Determination of chlorophyll a content
- DIN EN 17899:2025-04 Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality
- ISO 29841:2009/Amd 1:2016 Vegetable fats and oils — Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) — Amendment 1
- KS I ISO 10260-2006(2016) 수질-생화학적 지표 측정-농축 엽록소 a의 분광 측정
- KS I ISO 10260(2021 Confirm)(2021) 수질-생화학적 지표 측정-농축 엽록소 a의 분광 측정
- KS I ISO 10260-2006(2021) 수질-생화학적 지표 측정-농축 엽록소 a의 분광 측정
- ISO 10260:1992 Water quality — Measurement of biochemical parameters — Spectrometric determination of the chlorophyll-a concentration
- BS ISO 10260:1992 Water quality. Measurement of biochemical parameters. Spectrometric determination of the chlorophyll-a concentration
- BS ISO 29841:2009 Vegetable fats and oils. Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins)
- BS EN ISO 29841:2014 Vegetable fats and oils. Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins)
- DIN EN ISO 29841:2016-10 Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009 + AMD 1:2016) (includes Amendment :2016)
- ГОСТ 17.1.4.02-90 Вода. Методика спектрофотометрического определения хлорофилла а
- EN ISO 29841:2014 Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009)
- GB/T 22182-2025 油菜籽叶绿素含量的测定 分光光度计法
- EN ISO 29841:2014/A1:2016 Vegetable fats and oils - Determination of the degradation products of chlorophylls a and a' (pheophytins a, a' and pyropheophytins) (ISO 29841:2009/AMD 1:2016)
常见问题
叶绿素A测定检测需要多长时间?
通常7-15个工作日,具体视样品数量及萃取方法而定,大批量样品或需复杂前处理的样品周期可能延长。
叶绿素A测定检测费用大概多少?
根据检测项目数量确定,单一样品的基础浓度测定费用较低,若需进行色素组分全分析或加急处理则费用相应增加。
叶绿素A测定检测报告有什么用途?
可用于质量评估、项目验收,中析检测中心旗下实验室拥有CMA、CNAS、ISO等检验检测资质,报告具备社会证明作用。
叶绿素A测定采样时需要注意什么?
水样采集后应立即避光保存,使用滤膜富集后需低温冷冻,并尽快加入萃取剂,以防色素降解影响结果准确性。
总结
叶绿素A测定是对水体、藻类及沉积物中叶绿素A含量进行定量分析以评估水体富营养化程度的检测项目。中析检测中心旗下实验室拥有CMA、CNAS、ISO等检验检测资质。检测报告可用于环境监测、水质评价及科研项目验收。